RT Journal Article
T1 Development of a Fluorescent Bodipy Probe for Visualization of the Serotonin 5-HT1A Receptor in Native Cells of the Immune System
A1 Hernández Torres, Gloria
A1 Enríquez Palacios, Ernesto
A1 Mecha Rodríguez, Miriam
A1 Feliú Martínez, Ana
A1 Rueda Zubiaurre, Ainoa
A1 Angelina Querencias, Alba
A1 Martín Fontecha, María del Mar
A1 Palomares, Oscar
A1 Guaza Rodríguez, Carmen
A1 Peña Cabrera, Eduardo
A1 López Rodríguez, María L.
A1 Ortega Gutiérrez, Silvia
A1 Martín De La Cruz, Leticia
AB Serotonin (5-HT) modulates key aspects of the immune system. However, its precise function and the receptors involved in the observed effects have remained elusive. Among the different serotonin receptors, 5-HT1A plays an important role in the immune system given its presence in cells involved in both the innate and adaptive immune responses, but its actual levels of expression under different conditions have not been comprehensively studied due to the lack of suitable tools. To further clarify the role of 5-HT1A receptor in the immune system, we have developed a fluorescent small molecule probe that enables the direct study of the receptor levels in native cells. This probe allows direct profiling of the receptor expression in immune cells using flow cytometry. Our results show that important subsets of immune cells including human monocytes and dendritic cells express functional 5-HT1A and that its activation is associated with anti-inflammatory signaling. Furthermore, application of the probe to the experimental autoimmune encephalomyelitis model of multiple sclerosis demonstrates its potential to detect the specific overexpression of the 5-HT1A receptor in CD4+ T cells. Accordingly, the probe reported herein represents a useful tool whose use can be extended to study the levels of 5-HT1A receptor in ex vivo samples of different immune system conditions.
PB American Chemical Society (ACS)
SN 1043-1802
YR 2018
FD 2018-05-07
LK https://hdl.handle.net/20.500.14352/12192
UL https://hdl.handle.net/20.500.14352/12192
LA eng
NO The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.bioconjchem.8b00228.Received: March 30, 2018; Revised: May 4, 2018; Published: May 7, 2018
NO Ministerio de Economía y Competitividad (MINECO)
NO Red Española de Esclerosis Múltiple
NO CONACyT
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 1 sept 2024