RT Journal Article
T1 Exploring the potential mechanism of action of piperine against candida albicans and targeting its virulence factors
A1 Bravo Chaucanés, Claudia Patricia
A1 Chitiva, Luis Carlos
A1 Vargas Casanova, Yerly
A1 Diaz Santoyo, Valentina
A1 Hernández, Andrea Ximena
A1 Costa, Geison M.
A1 Parra Giraldo, Claudia Marcela
AB Plant-derived compounds have proven to be a source of inspiration for new drugs. In this study, piperine isolated from the fruits of Piper nigrum showed anti-Candida activity. Furthermore, the mechanisms of action of piperine and its impact on virulence factors in Candida albicans, which have not been comprehensively understood, were also assessed. Initially, piperine suppressed the hyphal transition in both liquid and solid media, hindered biofilm formation, and resulted in observable cell distortions in scanning electron microscope (SEM) samples, for both fluconazole-sensitive and fluconazole-resistant C. albicans strains. Additionally, the morphogenetic switches triggered by piperine were found to rely on the activity of mutant C. albicans strains. Secondly, piperine treatment increased cell membrane permeability and disrupted mitochondrial membrane potential, as evidenced by propidium iodine and Rhodamine 123 staining, respectively. Moreover, it induced the accumulation of intracellular reactive oxygen species in C. albicans. Synergy was obtained between the piperine and the fluconazole against the fluconazole-sensitive strain. Interestingly, there were no hemolytic effects of piperine, and it resulted in reduced cytotoxicity on fibroblast cells at low concentrations. The results suggest that piperine could have a dual mode of action inhibiting virulence factors and modulating cellular processes, leading to cell death in C. albicans.
PB MDPI
YR 2023
FD 2023-11-30
LK https://hdl.handle.net/20.500.14352/104561
UL https://hdl.handle.net/20.500.14352/104561
LA eng
NO Bravo-Chaucanés CP, Chitiva LC, Vargas-Casanova Y, Diaz-Santoyo V, Hernández AX, Costa GM, Parra-Giraldo CM. Exploring the Potential Mechanism of Action of Piperine against Candida albicans and Targeting Its Virulence Factors. Biomolecules. 2023;13(12):1729.
NO 2023 Descuento MDPIFunding:Convocatoria Ecosistema Científico-Colombia Científico “Generación de alternativas terapéuticas en cáncer a partir de plantas a través de procesos de investigación y desarrollo transnacional, articulados en sistemas de valor sostenibles ambiental y económicamente”,patrocinado por el Banco Mundial (contrato No. FP44842-221-2018).Convocatoria del Fondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías, en el marco del Programa de Becas de Excelencia Doctoral del Bicentenario.
NO Pontificia Universidad Javeriana
NO Ministerio de Ciencia Tecnología e Innovación (Colombia)
NO Ministerio de Educación Nacional (Colombia)
NO Ministerio de Comercio, Industria y Turismo (Colombia)
NO ICETEX
DS Docta Complutense
RD 17 abr 2025