RT Journal Article
T1 Design and characterization of an ocular topical liposomal preparation to replenish the lipids of the tear film
A1 Vicario De La Torre, Marta
A1 Benítez Del Castillo Sánchez, José Manuel
A1 Vico Ruiz, Eva
A1 Guzmán Pastor, Manuel
A1 Heras Polo, Beatriz De Las
A1 Herrero Vanrell, María Del Rocío
A1 Molina Martínez, Irene Teresa
AB Purpose: Dry eye (DE) includes a group of diseases related to tear film disorders. Current trends for DE therapy focus on providing lipid components to replace the damaged lipid layer. Formulations that contain aqueous and mucin like compounds may have additional therapeutic benefits for DE patients. The aim of this work was to design and evaluate novel formulations having the potential to become topical treatment for DE.Methods: Unpreserved liposomal formulations composed of phosphatidylcholine (PC), cholesterol, and α tocopherol (vit E) were prepared by the thin-film hydration technique. Formulations were characterized in terms of liposome size, pH, surface tension, osmolarity, and viscosity. In vitro tolerance assays were performed on macrophage, human corneal and conjunctival cell lines at short and long term exposures. In vivo ocular tolerance was studied after instillation of the formulation.Results: The mean liposome size was less than 1 μm and surface tension <30 50 mN/m for all formulations. The final liposomal formulation (PC:cholesterol:vit E in a ratio 8:1:0.8) had physiological values of pH (6.45 ± 0.09), osmolarity (289.43 ± 3.28 mOsm), and viscosity (1.82 ± 0.02 mPa·s). Cell viability was greater than 80% in the corneal and conjunctival cells. This formulation was well tolerated by experimental animals.Conclusions: The unpreserved liposomal formulation has suitable properties to be administered by topical ophthalmic route. The liposome-based artificial tear had good in vitro and in vivo tolerance responses. This formulation composed of a combination of liposomes and bioadhesive polymers may be employed successfully as a tear film substitute in DE therapy.
PB Association for Research in Vision and Ophthalmology (ARVO)
SN 1552-5783
YR 2014
FD 2014-12
LK https://hdl.handle.net/20.500.14352/34087
UL https://hdl.handle.net/20.500.14352/34087
LA eng
NO Vicario De La Torre, M., Benítez Del Castillo Sánchez, J. M., Vico Ruiz, E. et al. «Design and Characterization of an Ocular Topical Liposomal Preparation to Replenish the Lipids of the Tear Film». Investigative Ophthalmology & Visual Science, vol. 55, n.o 12, diciembre de 2014, pp. 7839-47. DOI.org (Crossref), https://doi.org/10.1167/iovs.14-14700.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Instituto de Salud Carlos III
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 6 abr 2025