RT Journal Article
T1 PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity
A1 Pardo, Mercedes
A1 Monteoliva Díaz, Lucía
A1 Vázquez, Paloma
A1 Martínez, Raquel
A1 Molero Martín-Portugués, María Gloria
A1 Nombela Cano, César
A1 Gil, Concha
AB Pst1p was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. ECM33 encodes a protein homologous to Pst1p, and both of them display typical features of GPI-anchored proteins and a characteristic receptor L-domain. Pst1p and Ecm33p are both localized to the cell surface, Pst1p being at the cell membrane and possibly also in the periplasmic space. Here, the characterization of pst1D, ecm33 and pst1 ecm33 mutants is described. Deletion of ECM33 leads to a weakened cell wall, and this defect is further aggravated by simultaneous deletion of PST1. As a result, the ecm33 mutant displays increased levels of activated Slt2p, the MAP kinase of the cell integrity pathway, and relies on a functional Slt2-mediated cell integrity pathway to ensure viability. Analyses of model glycosylated proteins show glycosylation defects in the ecm33 mutant. Ecm33p is also important for proper cell wall ultrastructure organization and, furthermore, for the correct assembly of the mannoprotein outer layer of the cell wall. Pst1p seems to act in the compensatory mechanism activated upon cell wall damage and, in these conditions, may partially substitute for Ecm33p.
PB Microbiology Society
SN 1350-0872
YR 2004
FD 2004
LK https://hdl.handle.net/20.500.14352/95408
UL https://hdl.handle.net/20.500.14352/95408
LA eng
NO Pardo M. PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity. Microbiology 2004;150:4157–70. https://doi.org/10.1099/mic.0.26924-0.
NO Comisión Interministerial de Ciencia y Tecnología
NO Comunidad de Madrid
DS Docta Complutense
RD 19 dic 2025