RT Journal Article
T1 Nanoceria and bulk cerium oxide effects on the germinationof Asplenium adiantum-nigrum spores
A1 Gómez Garay, Aranzazu
A1 Pintos López, Beatriz
A1 Manzanera, José Antonio
A1 Prada Moral, María Carmen
A1 Martín Calvarro, Luisa
A1 Gabriel Y Galán Moris, José María
AB Aim of the study: The effect of cerium oxide engineered nanoparticles on the spore germination of the fern Asplenium adiantumnigrum.Area of study: France, Britanny Region, Finistére Department, Plougonvelin, in rocks near the sea.Material and methods: Asplenium spores were cultured in vitro on agar medium with Nano-CeO2 (less than 25 nm particle size) and bulk-CeO2. The addition of each nano- and bulk particles ranged from 0 to 3000 mg L–1. Observations on rhizoidal and prothallial cells during first stages of gametophyte development were made. The No-Observed-Adverse-Effect concentration (NOAEC) and Lowest-Observed-Adverse-Effect-Concentration (LOEC) values for spore germination rate data were analyzed.Main results: Germination was speeded up by 100 to 2000 mg L–1 nanoceria, while bulk cerium oxide had the same effect for 500 to 2000 mg L–1 concentrations. Present results showed cellular damage in the protonema while rhizoid cells seemed not to be affected, as growth and membrane integrity remained.Research highlights: Both nanosized and bulk cerium oxide are toxic for the fern Asplenium adiantum-nigrum, although diverse toxicity patterns were shown for both materials. Diverse toxic effects have been observed: chloroplast membrane damage and lysis, cell wall and membrane disruption which leads to cell lysis; and alterations in morphology and development.
PB INIA
SN 2171-5068
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/23272
UL https://hdl.handle.net/20.500.14352/23272
LA eng
DS Docta Complutense
RD 12 abr 2025