RT Journal Article
T1 Parathyroid hormone-related protein (107-111) improves the bone regeneration potential of gelatin-glutaraldehyde biopolymer-coated hydroxyapatite
A1 Lozano Borregón, Daniel
A1 Sánchez Salcedo, Sandra
A1 Portal Núñez, Sergio
A1 Vila, Mercedes
A1 López Herradón, Ana
A1 Ardura, Juan A.
A1 Mulero, Francisca
A1 Gómez Barrena, Enrique
A1 Vallet Regí, María Dulce Nombre
A1 Esbrit, Pedro
AB Biopolymer-coated nanocrystalline hydroxyapatite (HA) made as macroporous foams which are degradable and flexible are promising candidates as orthopaedic implants. The C-terminal (107-111) epitope of parathyroid hormone-related protein (PTHrP) exhibits osteogenic properties. The main aim of this study was to evaluate whether PTHrP (107-111) loading into gelatin-glutaraldehyde biopolymer-coated HA (HA(GIu)) scaffolds would produce an optimal biomaterial for tissue engineering applications. HA(GIu) scaffolds with and without PTHrP (107-111) were implanted into a cavitary defect performed in both distal tibial metaphysis of adult rats. Animals were sacrificed after 4 weeks for histological, microcomputerized tomography and gene expression analysis of the callus. At this time, bone healing occurred only in the presence of PTHrP (107-111)-containing HA(Giu) implant, related to an increase in bone volume/tissue volume and trabecular thickness, cortical thickness and gene expression of osteocalcin and vascular cell adhesion molecule I, but a decreased gene expression of Wnt inhibitors, SOST and dickkopf homolog 1. The autonomous osteogenic effect of the PTHrP (107-111)-loaded HA(Giu) scaffolds was confirmed in mouse and human osteoblastic cell cultures. Our findings demonstrate the advantage of loading PTHrP (107-111) into degradable HA(GIu) scaffolds for achieving an optimal biomaterial that is promising for low load bearing clinical applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
PB Elsevier
SN 1742-7061
YR 2014
FD 2014-07
LK https://hdl.handle.net/20.500.14352/35384
UL https://hdl.handle.net/20.500.14352/35384
LA spa
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)	RESEARCHER ID N-4501-2014  (Sandra Sánchez Salcedo)ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)RESEARCHER ID B-5081-2017  (Daniel Lozano Borregón)ORCID 0000-0001-5902-9201 (Daniel Lozano Borregón)
NO Ministerio de Economia y Competitividad (MINECO)
NO Ministerio de Ciencia e Innovacion (MICINN)
NO Ministerio de Economia y Competitividad (RE-TICEF)
NO Comunidad de Madrid
NO RETICEF
DS Docta Complutense
RD 17 abr 2025