RT Journal Article T1 Biocompatibility evaluation of pH and glutathione-responsive nanohydrogels after intravenous administration A1 Pérez, Elena A1 Olmo López, Rosa María A1 Teijón López, César A1 Muñiz Hernando, Enriqueta A1 Montero, Nuria A1 Teijón Rivera, José María A1 Blanco Gaitán, María Dolores AB Nanotoxicology has emerged as an important subdiscipline of nanotechnology due to the new healthy risks associated with the use of nanosystems for therapy and diagnostic. The biocompatibility of four stimuli-responsive nanohydrogel (NG) formulations based on different proportions of N-isopropylacrylamide (NIPA), N-hydroxyethyl acrylamide (HEAA) and 2-acrylamidoethyl carbamate (2AAECM), and cross-linked with N,N-cystaminebisacrylamide (CBA) or N-methylenebisacrylamide (NMBA) has been evaluated after intravenous injection in Wistar rats. All nanohydrogels were pH-sensitive, and those with CBA were also glutathione-responsive. Haematological and coagulation parameters revealed most nanogel formulations did not cause modification, only the NHA 80/15/5-CBA formulation induced a transitory light increase in platelets. Prothrombin time was in the reference normal range, there were no modifications of fibrinogen concentration and an increase in antithrombin III was observed on the last day of the study. Blood biochemical parameters such as AST, ALT, ALP, BUN, and creatinine were in the standard range for rats. The activity of enzyme antioxidant defences (SOD, CAT and GSSG-R) and total glutathione were evaluated in liver, kidney and spleen samples. Nanohydrogels cross-linked with the disulphide reducible CBA-cross-linker caused a decrease in GSSG/GSH content and an increase in GSSG-R activity in the spleen. The antioxidant response is also reflected by modifications of SOD activity in liver and kidney of NHA 80/15/5-CBA and NHA 80/10/10-NMBA groups. Histology showed no tissue damage, inflammation or morphological change in liver, kidney and spleen. Overall, the results demonstrated modifications of antioxidant defences; however, no acute or very significant changes in biomarkers of liver or kidney damage were observed. PB Elsevier SN 0927-7765 YR 2015 FD 2015-12-01 LK https://hdl.handle.net/20.500.14352/23187 UL https://hdl.handle.net/20.500.14352/23187 LA eng NO Pérez, E., Olmo López, R. M., Teijón López, C. et al. «Biocompatibility Evaluation of pH and Glutathione-Responsive Nanohydrogels after Intravenous Administration». Colloids and Surfaces B: Biointerfaces, vol. 136, diciembre de 2015, pp. 222-31. Crossref, https://doi.org/10.1016/j.colsurfb.2015.09.017. NO Universidad Complutense de Madrid NO Universidad Complutense de Madrid/Banco de Santander DS Docta Complutense RD 10 abr 2025