RT Journal Article
T1 Improved Measurement of Elastic Properties of Cells by Micropipette Aspiration and Its Application to Lymphocytes
A1 Esteban Manzanares, Gustavo
A1 González Bermúdez, Blanca
A1 Cruces, Julia
A1 Fuente del Rey, Mónica de la
A1 Li, Qingxuan
A1 Guinea, Gustavo V.
A1 Pérez Rigueiro, José
A1 Elices Calafat, Manuel
A1 Plaza, Gustavo R.
AB Mechanical deformability of cells is an important property for their function and development, as well as a useful marker of cell state. The classical technique of micropipette aspiration allows single-cell studies and we provide here a method to measure the two basic mechanical parameters, elastic modulus and Poisson’s ratio. The proposed method, developed from finite-element analysis of micropipette aspiration experiments, may be implemented in future technologies for the automated measurement of mechanical properties of cells, based on the micropipette aspiration technique or on the cell transit through flow constrictions. We applied this method to measure the elastic parameters of lymphocytes, in which the mechanical properties depend on their activation state. Additionally, we discuss in this work the accuracy of previous models to estimate the elastic modulus of cells, in particular the analytical model by Theret et al., widely used in the field. We show the necessity of using an improved model, taking into account the finite size of the cells, to obtain new insights that may remain hidden otherwise.
PB Springer Link
SN 0090-6964
YR 2017
FD 2017-05
LK https://hdl.handle.net/20.500.14352/18009
UL https://hdl.handle.net/20.500.14352/18009
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 11 abr 2025