RT Journal Article
T1 BCEPS: A Web Server to Predict Linear B Cell Epitopes with Enhanced Immunogenicity and Cross-Reactivity
A1 Ras Carmona, Álvaro
A1 Peláez Prestel, Héctor Fernando
A1 Lafuente Duarte, María Esther
A1 Reche Gallardo, Pedro Antonio
AB Prediction of linear B cell epitopes is of interest for the production of antigen-specific antibodies and the design of peptide-based vaccines. Here, we present BCEPS, a web server for predicting linear B cell epitopes tailored to select epitopes that are immunogenic and capable of inducing cross-reactive antibodies with native antigens. BCEPS implements various machine learning models trained on a dataset including 555 linearized conformational B cell epitopes that were mined from antibody–antigen protein structures. The best performing model, based on a support vector machine, reached an accuracy of 75.38% ± 5.02. In an independent dataset consisting of B cell epitopes retrieved from the Immune Epitope Database (IEDB), this model achieved an accuracy of 67.05%. In BCEPS, predicted epitopes can be ranked according to properties such as flexibility, accessibility and hydrophilicity, and with regard to immunogenicity, as judged by their predicted presentation by MHC II molecules. BCEPS also detects if predicted epitopes are located in ectodomains of membrane proteins and if they possess N-glycosylation sites hindering antibody recognition. Finally, we exemplified the use of BCEPS in the SARS-CoV-2 Spike protein, showing that it can identify B cell epitopes targeted by neutralizing antibodies.
PB MDPI
SN 2073-4409
YR 2021
FD 2021-10-14
LK https://hdl.handle.net/20.500.14352/5057
UL https://hdl.handle.net/20.500.14352/5057
LA eng
NO Ras Carmona, Á., Peláez Prestel, H. F., Lafuente Duarte, M. E. & Reche Gallardo, P. A. «BCEPS: A Web Server to Predict Linear B Cell Epitopes with Enhanced Immunogenicity and Cross-Reactivity». Cells, vol. 10, n.o 10, octubre de 2021, p. 2744. DOI.org (Crossref), https://doi.org/10.3390/cells10102744.
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 5 abr 2025