RT Journal Article
T1 Enzyme-Powered Gated Mesoporous Silica Nanomotors for On-Command Intracellular Payload Delivery
A1 Llopis-Lorente, Antoni
A1 García-Fernández, Alba
A1 Murillo-Cremaes, Nerea
A1 Hortelão, Ana
A1 Patiño, Tania
A1 Villalonga Santana, Reynaldo
A1 Sancenón, Félix
A1 Martínez-Máñez, Ramón
A1 Sánchez, Samuel
AB The introduction of stimuli-responsive cargo release capabilities on self-propelled micro- and nanomotors holds enormous potential in a number of applications in the biomedical field. Herein, we report the preparation of mesoporous silica nanoparticles gated with pH-responsive supramolecular nanovalves and equipped with urease enzymes which act as chemical engines to power the nanomotors. The nanoparticles are loaded with different cargo molecules ([Ru(bpy)3]Cl2 (bpy = 2,2′-bipyridine) or doxorubicin), grafted with benzimidazole groups on the outer surface, and capped by the formation of inclusion complexes between benzimidazole and cyclodextrin-modified urease. The nanomotor exhibits enhanced Brownian motion in the presence of urea. Moreover, no cargo is released at neutral pH, even in the presence of the biofuel urea, due to the blockage of the pores by the bulky benzimidazole:cyclodextrin-urease caps. Cargo delivery is only triggered on-command at acidic pH due to the protonation of benzimidazole groups, the dethreading of the supramolecular nanovalves, and the subsequent uncapping of the nanoparticles. Studies with HeLa cells indicate that the presence of biofuel urea enhances nanoparticle internalization and both [Ru(bpy)3]Cl2 or doxorubicin intracellular release due to the acidity of lysosomal compartments.Gated enzyme-powered nanomotors shown here display some of the requirements for ideal drug delivery carriers such as the capacity to self-propel and the ability to “sense” the environment and deliver the payload on demand in response to predefined stimuli.
PB American Chemical Society
SN 1936-0851
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/92666
UL https://hdl.handle.net/20.500.14352/92666
LA eng
NO Llopis-Lorente, A., García-Fernández, A., Murillo-Cremaes, N., Hortelao, A., Patiño, T., Villalonga, R., Sancenón, F., Martinez-Manez, R., Sanchez, S. (2019) Enzyme-powered gated mesoporous silica nanomotors for on-command intracellular payload delivery. ACS Nano 13: 12171-12183.
NO “La Caixa” Banking Foundation
NO Ministerio de Economía y Competitividad (España)
NO BBVA Foundation
NO Generalitat Valenciana
NO Generalitat de Catalunya
NO European Commission
DS Docta Complutense
RD 15 abr 2025