RT Journal Article
T1 Direct detection of Brettanomyces bruxellensis in wine by PCR targeting the vinylphenol reductase gene
A1 Benito Vázquez, Iván
A1 Belda Aguilar, Ignacio
A1 Ruiz Ruiz, Javier
A1 Vicente Sánchez, Javier
A1 Navascués, Eva
A1 Marquina Díaz, Domingo
A1 Santos de la Sen, Antonio
AB Brettanomyces bruxellensis is among the main spoilage yeasts in wine usually found in oak barrels. As routine, laboratories at wineries use selective-differential culture media to detect this yeast. Nevertheless, it is widely recognized that other microbial species can grow on these media, getting false positive signals. In this work, we have developed a conventional PCR method based on the vinylphenol reductase gene (VPR1) of B. bruxellensis applied to wines after a polyvinylpyrrolidone-based pre-treatment (7% w/v) and that allowed us to reach a low detection limit, up to 102 UFC/mL of wine. The procedure was conceived without time-consuming DNA extraction steps, simplifying the methodology and making it easy for applying in wineries.
PB Elsevier
SN 0023-6438
YR 2020
FD 2020-10-03
LK https://hdl.handle.net/20.500.14352/8134
UL https://hdl.handle.net/20.500.14352/8134
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Pago de Carraovejas SLU
DS Docta Complutense
RD 6 may 2024