RT Book, Section
T1 Intracellular calcium recording and immunostaining of single neural populations in vitro
A1 Serrano-López, Julia
A1 Llorente-Sáez, Celia
A1 Pérez Sen, Raquel
A1 García Delicado, Esmerilda
A1 Ortega De La O, Felipe
A1 Gómez Villafuertes, María Rosa
A2 Ortega De La O, Felipe
AB Calcium plays a pivotal role as an intracellular messenger, eliciting a diverse array of cellular responses. One of the most common calcium-sensitive fluorescent indicators is the ratiometric dye Fura-2. The primary advantage of using ratiometric dyes lies in their independence from factors, such as illumination intensity, photobleaching, dye concentration, and focal alterations, among others. This independence allows for the determination of intracellular calcium concentration free from these interfering variables. In this protocol, we describe the utilization of Fura-2 to assess intracellular calcium elevations in cultures of neural stem cells using video-microscopy equipment. We have previously applied this methodology, commonly referred to as calcium imaging, to investigate intracellular calcium dynamics triggered by the activation of purinergic receptors in a variety of neural populations (Gomez-Villafuertes et al., Cell Transplant 24(8):1493–1509,  2015; Hervas et al., J Neurosci Res 73(3):384–399, 2003; Diaz-Hernandez et al., J Cell Sci 121(Pt 22):  3717–3728, 2008; Carrasquero et al., J Neurochem 110(3):879–889, 2009; Gomez Villafuertes et al.,  FEBS J 276(18):5307–5325, 2009).
PB Springer US
SN 9781071643853
SN 9781071643860
SN 1064-3745
SN 1940-6029
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/128606
UL https://hdl.handle.net/20.500.14352/128606
LA eng
NO Serrano-López, J., Llorente-Sáez, C., Pérez-Sen, R., Delicado, E. G., Ortega, F., & Gómez-Villafuertes, R. (2025). Intracellular Calcium Recording and Immunostaining of Single Neural Populations In Vitro. Methods in molecular biology (Clifton, N.J.), 2899, 275–287. https://doi.org/10.1007/978-1-0716-4386-0_19
NO Ministerio de Ciencia e Innovación y Universidades (España)
NO Ministerio de Ciencia e Innovación (España)
NO Red de Excelencia Consolider-Ingenio Spanish Ion Channel Initiative
NO European Commission
NO Banco Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 19 mar 2026