RT Journal Article
T1 The Heterotrimeric Laminin Coiled-Coil Domain Exerts Anti-Adhesive Effects and Induces a Pro-Invasive Phenotype
A1 Santos-Valle, Patricia
A1 Guijarro-Muñoz, Irene
A1 Cuesta Martínez, Ángel
A1 Alonso-Camino, Vanesa
A1 Villate, Maider
A1 Álvarez-Cienfuegos, Ana
A1 Blanco, Francisco J.
A1 Sanz, Laura
A1 Álvarez-Vallina, Luis
AB Laminins are large heterotrimeric cross-shaped extracellular matrix glycoproteins with terminal globular domains and a coiled-coil region through which the three chains are assembled and covalently linked. Laminins are key components of basement membranes, and they serve as attachment sites for cell adhesion, migration and proliferation. In this work, we produced a recombinant fragment comprising the entire laminin coiled-coil of the α1-, β1-, and γ1-chains that assemble into a stable heterotrimeric coiled-coil structure independently of the rest of the molecule. This domain was biologically active and not only failed to serve as a substrate for cell attachment, spreading and focal adhesion formation but also inhibited cell adhesion to laminin when added to cells in a soluble form at the time of seeding. Furthermore, gene array expression profiling in cells cultured in the presence of the laminin coiled-coil domain revealed up-regulation of genes involved in cell motility and invasion. These findings were confirmed by real-time quantitative PCR and zymography assays. In conclusion, this study shows for the first time that the laminin coiled-coil domain displays anti-adhesive functions and has potential implications for cell migration during matrix remodeling.
PB PLOS
SN 1932-6203
YR 2012
FD 2012-06-19
LK https://hdl.handle.net/20.500.14352/115530
UL https://hdl.handle.net/20.500.14352/115530
LA eng
NO Santos-Valle P, Guijarro-Mun˜oz I, Cuesta A´ M, Alonso-Camino V, Villate M, et al. (2012) The Heterotrimeric Laminin Coiled-Coil Domain Exerts AntiAdhesive Effects and Induces a Pro-Invasive Phenotype. PLoS ONE 7(6): e39097. doi:10.1371/journal.pone.0039097
NO Ministerio de Ciencia e Innovacion (España)
NO Comunidad de Madrid
NO FEDER
NO Instituto de Salud Carlos III
NO Hospital Universitario Puerta de Hierro
DS Docta Complutense
RD 26 dic 2025