RT Journal Article
T1 Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats
A1 Mas Capdevila, Anna
A1 Iglesias Carres, Lisard
A1 Arola-Arnal, Anna
A1 Aragonès, Gerard
A1 Aleixandre De Artiñano, María Amaya
A1 Bravo, Francisca
A1 Muguerza, Begoña
AB AVFQHNCQE is an antihypertensive nonapeptide obtained from a chicken foot protein hydrolysate. The present study aims to investigate the mechanisms involved in its blood pressure (BP)-lowering effect. Male (17–20 weeks old) spontaneously hypertensive rats (SHR) were used in this study. Rats were divided into two groups and orally administered water or 10 mg/kg body weight (bw) AVFQHNCQE. One hour post administration, animals of both groups were intra peritoneally treated with 1 mL of saline or with 1 mL of saline containing 30 mg/kg bw Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, or with 1 mL of saline containing 5 mg/kg bw indomethacin, which is an inhibitor of prostacyclin synthesis (n = 6 per group). Systolic BP was recorded before oral administration and six hours after oral administration. In an additional experiment, SHR were administered water or 10 mg/kg bw AVFQHNCQE (n = 6 per group) and sacrificed six hours post administration to study the mechanisms underlying the peptide anti-hypertensive effect. Moreover, the relaxation caused by AVFQHNCQE in isolated aortic rings from Sprague Dawley rats was evaluated. The BP-lowering effect of the peptide was not changed after indomethacin administration but was completely abolished by L-NAME, which demonstrates that its anti-hypertensive effect is mediated by changes in endothelium-derived NO availability. In addition, AVFQHNCQE administration downregulated aortic gene expression of the vasoconstrictor factor endothelin-1 and the endothelial major free radical producer NADPH. Moreover, while no changes in plasma ACE activity were observed after its administration, liver GSH levels were higher in the peptide treated group than in the water group, which demonstrates that AVFQHNCQE presents antioxidant properties.
PB MDPI
SN 2072-6643
YR 2019
FD 2019-01-22
LK https://hdl.handle.net/20.500.14352/12744
UL https://hdl.handle.net/20.500.14352/12744
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 6 may 2024