RT Journal Article
T1 Colonial architecture and growth dynamics of Staphylococcus aureus resistant to methicillin
A1 Gómez Aguado, Fernando
A1 Gómez-Lus Centelles, María Luisa
A1 Corcuera, María Teresa
A1 Alou Cervera, Luis
A1 Alonso, María José
A1 Sevillano Fernández, David
A1 Val, Daniel
A1 Palmeiro, Alejandra
A1 Iglesias, Nuria
A1 Prieto Prieto, José
AB The aim of the study was to explore the structure and growth dynamics of Staphylococcus aureus resistant to methicillin (MRSA) colonies using semithin sections visualized by light microscope. One S. aureus susceptible to methicillin (MSSA) and one MRSA clinical strains were studied. Colonies in agar plates were embedded in epoxy resin after each incubation period (24 h and 48 h) at 37 degrees . Semithin sections of 0.5 µm were stained with toluidine blue and visualized by light microscope. Microscopically, no structural differences were observed between SASM and SARM colonies but differences were observed in both strains between 24 and 48 h incubation periods. Colonies showed two layers clearly differentiated at 24 h independently of the resistance to methicillin: (A) one basal layer with high density of population in contact with culture media, and (B) one superficial layer with a lower density of population. Colonies showed four layers at 48 h: (A) one basal layer with high density of population; (B) one clear layer constituted by very degraded bacterial remains in which can be observed cocci dispersed with their dyeing properties; (C) one mixed layer constituted by viable bacteria and little degraded bacterial remains (D) one superficial layer with a lower density of population than basal layer. Colonial architecture is a complex and time-dependent process.
PB Sociedad Española de Quimioterapia
YR 2009
FD 2009-12
LK https://hdl.handle.net/20.500.14352/107127
UL https://hdl.handle.net/20.500.14352/107127
LA eng
NO Gómez-Aguado F, Gómez-Lus ML, Corcuera MT, Alou L, Alonso MJ, Sevillano D, Val D, Palmeiro A, Iglesias N, Prieto J. Colonial architecture and growth dynamics of Staphylococcus aureus resistant to methicillin. Rev Esp Quimioter. 2009 Dec;22(4):224-7
DS Docta Complutense
RD 15 jun 2025