%0 Journal Article
%A Moya-Cavas, Tamara
%A Navarro Villoslada, Fernando
%A Urraca Ruiz, Javier
%A Antonio Serrano, Luis
%A Orellana Moraleda, Guillermo
%A Moreno-Bondi, María Cruz
%A Moreno Bondi, María Cruz
%T Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads
%D 2023
%@ 0308-8146
%U https://hdl.handle.net/20.500.14352/91822
%X This work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 μg kg- 1, respectively. Recoveries were in the range of 94 % to 108 % (RSD < 6 %) for zearalenone and 92 % to 113 % (RSD < 5 %) for alternariol. The results were confirmed by HPLC-MS/MS.
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