RT Journal Article
T1 Carriage of Staphylococcus aureus by free-living wild animals in Spain
A1 Porrero, M Concepción
A1 Mentaberre, Gregorio
A1 Sánchez, Sergio
A1 Fernández Llario, Pedro
A1 Casas Díaz, Encarna
A1 Mateos García, Ana
A1 Vidal, Dolors
A1 Lavín, Santiago
A1 Domínguez Rodríguez, Lucas José
A1 Fernández-Garayzábal Fernández, José Francisco
AB The presence of methicillin-susceptible Staphylococcus aureus (MSSA) was analyzed in different free-living wild animals to assess the genetic diversity and predominant genotypes on each animal species. Samples were taken from the skin and/or nares, and isolates were characterized by spa typing, multilocus sequence typing (MLST) and antimicrobial susceptibility testing. The proportion of MSSA carriers were 5.00, 22.93, 19.78, and 17.67% in Eurasian griffon vulture, Iberian ibex, red deer, and wild boar, respectively (P = 0.057). A higher proportion of isolates (P = 0.000) were recovered from nasal samples (78.51%) than skin samples (21.49%), but the 9.26% of red deer and 18.25% of wild boar would have been undetected if only nasal samples had been tested. Sixty-three different spa types were identified, including 25 new spa types. The most common were t528 (43.59%) in Iberian ibex, t548 and t11212 (15.79% and 14.04%) in red deer, and t3750 (36.11%) in wild boar. By MLST, 27 STs were detected, of which 12 had not been described previously. The most frequent were ST581 for Iberian ibex (48.72%), ST425 for red deer (29.82%), and ST2328 for wild boar (42.36%). Isolates from Eurasian griffon vulture belong to ST133. Host specificity has been observed for the most frequent spa types and STs (P = 0.000). The highest resistance percentage was found against benzylpenicillin (average, 22.2%), although most of the S. aureus isolates were susceptible to all antimicrobial tested. Basically, MSSA isolates were different from those MRSA isolates previously detected in the same animal species.
PB American Society for Microbiology
SN 1098-5336
YR 2014
FD 2014-08
LK https://hdl.handle.net/20.500.14352/35276
UL https://hdl.handle.net/20.500.14352/35276
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Comunidad de Madrid
DS Docta Complutense
RD 27 abr 2024