RT Journal Article
T1 Gene characterization of nocturnin paralogues in goldfish: full coding sequences, structure, phylogeny and tissue expression
A1 Madera Sánchez, Diego
A1 Alonso-Gómez, Aitana
A1 Delgado Saavedra, María Jesús
A1 Valenciano González, Ana Isabel
A1 Alonso Gómez, Ángel Luis
AB The aim of this work is the full characterization of all the nocturnin (noc) paralogues expressed in a teleost, the goldfish. An in silico analysis of the evolutive origin of noc in Osteichthyes is performed, including the splicing variants and new paralogues appearing after teleostean 3R genomic duplication and the cyprinine 4Rc. After sequencing the full-length mRNA of goldfish, we obtained two isoforms for noc-a (noc-aa and noc-ab) with two splice variants (I and II), and only one for noc-b (noc-bb) with two transcripts (II and III). Using the splicing variant II, the prediction of the secondary and tertiary structures renders a well-conserved 3D distribution of four α-helices and nine β-sheets in the three noc isoforms. A synteny analysis based on the localization of noc genes in the patrilineal or matrilineal subgenomes and a phylogenetic tree of protein sequences were accomplished to stablish a classification and a long-lasting nomenclature of noc in goldfish, and valid to be extrapolated to allotetraploid Cyprininae. Finally, both goldfish and zebrafish showed a broad tissue expression of all the noc paralogues. Moreover, the enriched expression of specific paralogues in some tissues argues in favour of neo- or subfunctionalization.
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/105782
UL https://hdl.handle.net/20.500.14352/105782
LA eng
NO Int. J. Mol. Sci. 2024, 25(1), 54; https://doi.org/10.3390/ijms25010054
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 25 ago 2024