%0 Generic
%A Castillejo Pons, Pablo
%A Lobo, Eduardo 
%A Heinrich, Carla
%A Perdiguero Jiménez, Pedro
%A Frandsen, Paul
%A Rios-Touma, Blanca
%A Ballesteros Redondo, María Isabel
%T Assessment of diatom diversity in freshwater ecosystems of Ecuador using Nanopore-Based DNA Metabarcoding
%D 2023
%U https://hdl.handle.net/20.500.14352/107425
%X Our study aimed to compare the effectiveness of microscopic and DNA metabarcoding approaches in assessing the abundance and diversity of epilithic diatoms, while also exploring the suitability of metabarcoding as a reliable biomonitoring tool in Andean freshwater ecosystems. For this, 16 samples from the Guayllabamba River Basin in Ecuador were analyzed. Microscopic analysis involved the taxonomic identification of at least 600 valves under a light microscope. The molecular approach involved amplifying a rbcL region of approximately 1500 bp and conducting high-throughput DNA sequencing using Oxford Nanopore technology.
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