RT Journal Article
T1 Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida albicans
A1 Vaz, Catarina Oliveira
A1 Reales Calderón, José Antonio
A1 Pitarch Velasco, Aída
A1 Vellosillo, Perceval
A1 Trevisan Herraz, Marco
A1 Hernáez Sánchez, Maria Luisa
A1 Monteoliva Díaz, Lucía
A1 Gil García, Concha
AB Macrophages are involved in the primary human response to Candida albicans. After pathogen recognition, signaling pathways are activated, leading to the production of cytokines, chemokines, and antimicrobial peptides. ATP binding proteins are crucial for this regulation. Here, a quantitative proteomic and phosphoproteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. From a total of 547 nonredundant quantified proteins, 137 were ATP binding proteins and 59 were detected as differentially abundant. From the differentially abundant ATP-binding proteins, 6 were kinases (MAP2K2, SYK, STK3, MAP3K2, NDKA, and SRPK1), most of them involved in signaling pathways. Furthermore, 85 phosphopeptides were quantified. Macrophage proteomic alterations including an increase of protein synthesis with a consistent decrease in proteolysis were observed. Besides, macrophages showed changes in proteins of endosomal trafficking together with mitochondrial proteins, including some involved in the response to oxidative stress. Regarding cell death mechanisms, an increase of antiapoptotic over pro-apoptotic signals is suggested. Furthermore, a high pro-inflammatory response was detected, together with no upregulation of key mi-RNAs involved in the negative feedback of this response. These findings illustrate a strategy to deepen the knowledge of the complex interactions between the host and the clinically important pathogen C. albicans.
PB ACS
SN 1535-3893
YR 2019
FD 2019-04-15
LK https://hdl.handle.net/20.500.14352/13404
UL https://hdl.handle.net/20.500.14352/13404
LA eng
NO Vaz, C. O., Reales Calderón, J. A., Pitarch Velasco, A. et al. «Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida Albicans». Journal of Proteome Research, vol. 18, n.o 5, mayo de 2019, pp. 2139-59. DOI.org (Crossref), https://doi.org/10.1021/acs.jproteome.9b00032.
NO Unión Europea
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 29 abr 2025