RT Journal Article
T1 Atomic (HPLC-ICP-MS) and molecular mass spectrometry(ESI-Q-TOF) to study cis-platin interactions with serum proteins
A1 Esteban Fernández, Diego
A1 Montes Bayón, M.
A1 Blanco González, E.
A1 Gómez Gómez, María Milagros
A1 Palacios Corvillo, M. Antonia
A1 Sanz Medel, A.
AB The judicious use of cis-Pt as an intravenously administrable Pt(II) drug for chemotherapy requires the evaluation of its interactions with blood proteins. Therefore, the combined use of modern analytical chemical speciation and of analytical proteomics approaches to study these interactions is described here. The method involves incubation of cis-Pt with standard proteins and human serum samples. The separation of the proteins is conducted by liquid chromatography in an anion exchange column (Mono Q). Simultaneous molecular detection by UV absorption (280 nm) and elemental detection (195Pt) using inductively coupled plasma mass spectrometry (ICP-MS) are performed. Using this set-up, the effects of the incubation time as well as the drug concentration on cis-Pt interactions with transferrin, albumin and innmunoglobulin G were studied. In addition, the nature of interactions was also investigated by means of electrospray mass spectrometry (ESI-Q-TOF) of the intact protein. Transferrin and albumin showed different interactions, binding one and four cisplatin molecules, respectively. Also, some typical proteomic studies were initiated by tryptic digesting the transferrin and albumin cis-Pt complexes followed by capillary-LC-ICP-MS and ESI-Q-TOF parallel detection of the peptides obtained. The capLCICP-MS chromatogram provided clear evidence of Pt-containing peptides remaining after tryptic digestion.
PB RSC
SN 1364-5544 (Online) 0267-9477 (Print)
YR 2008
FD 2008
LK https://hdl.handle.net/20.500.14352/52021
UL https://hdl.handle.net/20.500.14352/52021
LA eng
NO Ministerio de Educación y Ciencia
NO Fondo Social Europeo for the contract of MMB
DS Docta Complutense
RD 13 may 2025