RT Journal Article
T1 A Simple Method to Measure Renal Function in Swine by the Plasma Clearance of Iohexol
A1 Luis-Lima, Sergio
A1 García-Contreras, Consolación
A1 Vázquez Gómez, Marta
A1 Astiz, Susana
A1 Carrara, Fabiola
A1 Gaspari, Flavio
A1 Negrín-Mena, Natalia
A1 Jiménez-Sosa, Alejandro
A1 Jiménez-Hernández, Hugo
A1 González Bulnes, Antonio
A1 Porrini, Esteban
AB There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = −47.909 + (1.176xCL1) − (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.
PB MDPI
SN 1422-0067
YR 2018
FD 2018-01-12
LK https://hdl.handle.net/20.500.14352/12523
UL https://hdl.handle.net/20.500.14352/12523
LA eng
NO Unión Europea. FP7
NO European Cooperation in Science and Technology (COST)
NO Ministerio de Economía y Competitividad (MINECO)/Programa Ramón y Cajal
NO Insituto de Salud Carlos III (ISCIII)
NO Red de Investigación Renal (REDINREN)
DS Docta Complutense
RD 9 abr 2025