RT Book, Section
T1 Evaluation of Mitochondrial Phagy (Mitophagy) in Human Non-small Adenocarcinoma Tumor Cells
A1 Alizadeh, Javad
A1 da Silva Rosa, Simone C.
A1 Cordani, Marco
A1 Ghavami, Saeid
A2 Turksen, Kursad
AB Non–small cell lung cancer (NSCLC) is a predominant form of lung cancer characterized by its aggressive nature and high mortality rate, primarily due to late-stage diagnosis and metastatic spread. Recent studies underscore the pivotal role of mitophagy, a selective form of autophagy targeting damaged or superfluous mitochondria, in cancer biology, including NSCLC. Mitophagy regulation may influence cancer cell survival, proliferation, and metastasis by modulating mitochondrial quality and cellular energy homeostasis. Herein, we present a comprehensive methodology developed in our laboratory for the evaluation of mitophagy in NSCLC tumor cells. Utilizing a combination of immunoblotting, immunocytochemistry, and fluorescent microscopy, we detail the steps to quantify early and late mitophagy markers and mitochondrial dynamics. Our findings highlight the potential of targeting mitophagy pathways as a novel therapeutic strategy in NSCLC, offering insights into the complex interplay between mitochondrial dysfunction and tumor progression. This study not only sheds light on the significance of mitophagy in NSCLC but also establishes a foundational approach for its investigation, paving way for future research in this critical area of cancer biology.
PB Springer
SN 9781071642672
SN 9781071642689
SN 1064-3745
YR 2024
FD 2024-04-13
LK https://hdl.handle.net/20.500.14352/118981
UL https://hdl.handle.net/20.500.14352/118981
LA eng
NO Alizadeh, J., Da Silva Rosa, S. C., Cordani, M., & Ghavami, S. (2024). Evaluation of mitochondrial phagy (Mitophagy) in human non-small adenocarcinoma tumor cells. En K. Turksen (Ed.), Autophagy in Development and Disease (Vol. 2879, pp. 261-273). Springer US. https://doi.org/10.1007/7651_2024_532
NO AcknowledgmentsSG conceptualized and designed the study. JA performed the experiments. SG and JA SCDR analyzed the data. JA and MC wrote the manuscript. SG, JA, SCDR, and MC reviewed and edited the manuscript. SG funded and supervised the experiments.FundingThis study was funded by Canercare Manitoba Foundation. JA was supported by CIHR Vaneir PhD studentship. SCDR was supported with a Postdoctoral Fellowship sponsored by the Canadian Institutes of Health and Research (CIHR), FRN 176508. MC was supported by grant RYC2021-031003I funded by MICIU/AEI/10.13039/501100011033 and, by European Union NextGenerationEU/PRTR.
NO Canercare Manitoba Foundation
NO Canadian Institutes of Health and Research (CIHR)
NO Unión Europea
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 6 abr 2025