RT Journal Article T1 Evidence of Leishmania infantum infection in rabbits (Oryctolagus cuniculus) in a natural area in Madrid, Spain A1 García Benzaquén, Nerea A1 Moreno, Inmaculada A1 Alvarez, Julio A1 Cruz Conty, María Luisa A1 Navarro Gómez, Alejandro A1 Pérez Sancho, Marta A1 Conty, María Luisa A1 Toraño, Alfredo A1 Prieto, Antonio A1 Domínguez, Mercedes A1 Álvarez Sánchez, Julio A1 Domínguez Rodríguez, Lucas José A1 Rodríguez Bertos, Antonio Manuel A1 García-Seco Romero, María Teresa AB Leishmaniasis is one of most important neglected zoonosis and remains endemic in at least 88 developing countries in the world. In addition, anthropogenic environmental changes in urban areas are leading to its emergency world-wide. Zoonotic leishmaniasis control might only be achieved by an integrated approach targeting both the human host and the animal reservoirs, which in certain sylvatic cycles are yet to be identified. Recently, hares have been pointed out as competent reservoirs of Leishmania infantum in Spain, but the role of other lagomorphs has not been clarified. Here, 69 rabbits (Oryctolagus cuniculus) from a natural area in Madrid in which a high density was present were analyzed using indirect (immunofluorescence antibody test, IFAT) and direct (PCR, culture) techniques. Fifty-seven (82.6%) of the animals were positive to at least one technique, with IFAT yielding the highest proportion of positive samples. L. infantum was isolated in 13% animals demonstrating the occurrence of infection in this setting. Our results suggest that rabbits could play a role of competent reservoir of L. infantum and demonstrate that the prevalence of infection is high in the analyzed area. PB Hindawi Publishing Corporation SN 2314-6133 YR 2014 FD 2014 LK https://hdl.handle.net/20.500.14352/35299 UL https://hdl.handle.net/20.500.14352/35299 LA eng DS Docta Complutense RD 15 may 2024