RT Journal Article
T1 Label-free bioanalysis of Leishmania infantum using refractive index tomography with partially coherent illumination
A1 Soto Rueda, Juan Manuel
A1 Rodrigo Martín-Romo, José Augusto
A1 Mas Zubiri, Alicia
A1 Domínguez Bernal, Gustavo Ramón
A1 Alieva Krasheninnikova, Tatiana
AB In this work, we report the use of refractive index (RI) tomography for quantitative analysis of unstained DH82 cell line infected with Leishmania infantum. The cell RI is reconstructed by using a modality of optical diffraction tomography technique that employs partially coherent illumination, thus enabling inherent compatibility with conventional wide-field microscopes. The experimental results demonstrate that the cell dry mass concentration (DMC) obtained from the RI allows for reliable detection and quantitative characterization of the infection and its temporal evolution. The RI provides important insight for studying morphological changes, particularly membrane blebbing linked to an apoptosis (cell death) process induced by the disease. Moreover, the results evidence that infected DH82 cells exhibit a higher DMC than healthy samples. These findings open up promising perspectives for clinical diagnosis of Leishmania.
PB WILEY‐VCH Verlag GmbH
SN 1864-063X
YR 2019
FD 2019-09
LK https://hdl.handle.net/20.500.14352/13769
UL https://hdl.handle.net/20.500.14352/13769
LA eng
NO © 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.The Ministerio de Economía y Competitividad is acknowledged for funding the project TEC2014-57394-P. This research was partially supported through a Santander-UCM 2018 project (PR75/18-21567) and by a grant from Comunidad de Madrid (Spain) PLATESA (S2013/ABI-2906). Alicia Mas was supported by a fellowship from Complutense University of Madrid-Santander.
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 5 abr 2025