RT Journal Article
T1 Carbon Nanohorns as a Scaffold for the Construction of Disposable Electrochemical Immunosensing Platforms. Application to the Determination of Fibrinogen in Human Plasma and Urine
A1 Ojeda Fernández, Irene
A1 Garcinuño, Belit
A1 Moreno Guzmán, María
A1 González Cortés, Araceli
A1 Yudasaka, Masako
A1 Iijima, Sumio
A1 Langa, Fernando
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB We describe in this work a novel electrochemical immunosensor design making use of carbon nanohorns (CNHs) as a scaffold for the preparation of disposable immunosensing platforms for the determination of fibrinogen (Fib). The approach involved the immobilization of Fib onto activated CNHs deposited on screen-printed carbon electrodes (SPCEs) and the implementation of an indirect competitive assay using anti-Fib labeled with horseradish peroxidase (HRP) and hydroquinone (HQ) as the redox mediator. Both CNHs and the Fib-CNHs covalent assembly were characterized by microscopic and electrochemical techniques. The different variables affecting the analytical performance of the amperometric immunosensing strategy were optimized. The calibration plot for Fib allowed a range of linearity between 0.1 and 100 μg/mL (r 2 = 0.994) and a detection limit of 58 ng/ mL to be achieved. The Fib-CNHs/SPCEs exhibited an excellent storage stability of at least 42 days. The developed immunosensor provides, in general, an analytical performance better than that reported for other Fib immunosensors and commercial ELISA kits. This simple and relatively low cost immunosensor configuration permitted the sensitive and selective determination of Fib in human plasma and urine
PB ACS
SN 0003-2700
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/91826
UL https://hdl.handle.net/20.500.14352/91826
LA eng
NO Anal. Chem. 2014, 86, 15, 7749–7756 Publication Date:July 7, 2014 https://doi.org/10.1021/ac501681n
NO Ministerio de Economía y ́Competitividad (España)
DS Docta Complutense
RD 20 ago 2024