RT Journal Article
T1 Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain
A1 Santín, Mónica
A1 Calero Bernal, Rafael
A1 Carmena, David
A1 Mateo Barrientos, Marta
A1 Balseiro, Ana
A1 Barral, Marta
A1 Lima Barbero, José Francisco
A1 Habela, Miguel Ángel
AB Microsporidia comprises a diverse group of obligate intracellular parasites that infect a broad range of invertebrates and vertebrates. Among Microsporidia, Enterocytozoon bieneusi is the most frequently detected species in humans and animals worldwide bringing into question the possible role of animal reservoirs in the epidemiology of this pathogen. Although E. bieneusi is an emerging zoonotic pathogen able to infect many domestic and wild mammals that could act as reservoir of infection for humans and other animals, only few studies have documented its occurrence in wild carnivores. To determine the occurrence of E. bieneusi in wild carnivores, we examined 190 wild carnivores collected from different locations in Spain. Twenty-five fecal samples (13.2%) from three host species (European badger, beech marten, and red fox) were E. bieneusi-positive by PCR. Nucleotide sequence analysis of the ITS region revealed a high degree of genetic diversity with a total of eight distinct genotypes including four known (PtEbIX, S5, S9, and WildBoar3) and four novel (EbCar1-EbCar4) genotypes identified. Phylogenetic analysis showed that the four novel genotypes (EbCar1-EbCar4), S5, S9, and WildBoar3 clustered within the previously designated zoonotic Group 1. Our results demonstrate that human-pathogenic genotypes are present in wild carnivores, corroborating their potential role as a source of human infection and environmental contamination.
PB Wiley
SN 1066-5234
YR 2017
FD 2017-12-22
LK https://hdl.handle.net/20.500.14352/98623
UL https://hdl.handle.net/20.500.14352/98623
LA eng
NO Santín, M., Calero-Bernal, R., Carmena, D., Mateo, M., Balseiro, A., Barral, M., Lima Barbero, J. F., & Habela, M. Á. (2018). Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain. The Journal of eukaryotic microbiology, 65(4), 468–474. https://doi.org/10.1111/jeu.12492
DS Docta Complutense
RD 10 sept 2024