RT Journal Article
T1 An Optical Technique for Mapping Microviscosity Dynamics in Cellular Organelles
A1 Chambers, Joseph E.
A1 Kubánková, Markéta
A1 Huber, Roland G.
A1 López Duarte, Ismael
A1 Avezov, Edward
A1 Bond, Peter J.
A1 Marciniak, Stefan J.
A1 Kuimova, Marina K.
AB Microscopic viscosity (microviscosity) is a key determinant of diffusion in the cell and defines the rate of biological processes occurring at the nanoscale, including enzyme-driven metabolism and protein folding. Here we establish a rotor-based organelle viscosity imaging (ROVI) methodology that enables real-time quantitative mapping of cell microviscosity. This approach uses environment-sensitive dyes termed molecular rotors, covalently linked to genetically encoded probes to provide compartment-specific microviscosity measurements via fluorescence lifetime imaging. ROVI visualized spatial and temporal dynamics of microviscosity with suborganellar resolution, reporting on a microviscosity difference of nearly an order of magnitude between subcellular compartments. In the mitochondrial matrix, ROVI revealed several striking findings: a broad heterogeneity of microviscosity among individual mitochondria, unparalleled resilience to osmotic stress, and real-time changes in microviscosity during mitochondrial depolarization. These findings demonstrate the use of ROVI to explore the biophysical mechanisms underlying cell biological processes.
PB American Chemical Society
SN 1936-0851
SN 1936-086X
YR 2018
FD 2018-04-12
LK https://hdl.handle.net/20.500.14352/115607
UL https://hdl.handle.net/20.500.14352/115607
LA eng
NO Chambers JE, Kubánková M, Huber RG, López-Duarte I, Avezov E, Bond PJ, et al. An optical technique for mapping microviscosity dynamics in cellular organelles. ACS Nano [Internet]. 22 de mayo de 2018 [citado 22 de enero de 2025];12(5):4398-407. Disponible en: https://pubs.acs.org/doi/10.1021/acsnano.8b00177
NO Alpha-1 Foundation
NO Engineering & Physical Sciences Research Council
DS Docta Complutense
RD 21 abr 2025