%0 Journal Article
%A Nguyen, Kim
%A Kubota, Miles
%A Del Arco, Jon
%A Feng, Chao
%A Singha, Monika
%A Beasley, Samantha
%A Sakr, Jasmine
%A Gandhi, Sunil P.
%A Blurton-Jones, Matthew
%A Fernández Lucas, Jesus
%A Spitale, Robert C.
%T A bump-hole strategy for increased stringency of cell-specific metabolic labeling of RNA
%D 2020
%@ 1554-8929
%U https://hdl.handle.net/20.500.14352/109478
%X Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.
%~