RT Journal Article
T1 A bump-hole strategy for increased stringency of cell-specific metabolic labeling of RNA
A1 Nguyen, Kim
A1 Kubota, Miles
A1 Del Arco, Jon
A1 Feng, Chao
A1 Singha, Monika
A1 Beasley, Samantha
A1 Sakr, Jasmine
A1 Gandhi, Sunil P.
A1 Blurton-Jones, Matthew
A1 Fernández Lucas, Jesus
A1 Spitale, Robert C.
AB Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.
PB American Chemical Society
SN 1554-8929
YR 2020
FD 2020-11
LK https://hdl.handle.net/20.500.14352/109478
UL https://hdl.handle.net/20.500.14352/109478
LA eng
NO Nguyen K, Kubota M, Del Arco J, Feng C, Singha M, Beasley S, Sakr J, Gandhi SP, Blurton-Jones M, Fernández Lucas J, Spitale RC. A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA. ACS Chem Biol 2020;15:3099–105. https://doi.org/10.1021/acschembio.0c00755.
NO RNA research in the Spitale lab is supported by startup funds from the University of California, Irvine, the NIH Director’s New Innovator Award (1DP2GM119164 RCS). S.B. is supported by an NIH training grant 5T32CA009054-40.
NO University of California (Berkeley)
DS Docta Complutense
RD 10 abr 2025