RT Journal Article
T1 Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA
A1 Escudero García-Calderón, José Antonio
A1 San Millán, Alvaro
A1 Gutiérrez, Belen
A1 Hidalgo, Laura
A1 La Ragione, Roberto M
A1 AbuOun, Manal
A1 Galimand, Marc
A1 Ferrándiz, María José
A1 Domínguez Rodríguez, Lucas José
A1 de la Campa, Adela G
A1 González Zorn, Bruno
AB Streptococcus suis is an emerging zoonotic pathogen. With the lack of an effective vaccine, antibiotics remain the main tool to fight infections caused by this pathogen. We have previously observed a reserpine-sensitive fluoroquinolone (FQ) efflux phenotype in this species. Here, SatAB and SmrA, two pumps belonging to the ATP binding cassette (ABC) and the major facilitator superfamily (MFS), respectively, have been analyzed in the fluoroquinolone-resistant clinical isolate BB1013. Genes encoding these pumps were overexpressed either constitutively or in the presence of ciprofloxacin in this strain. These genes could not be cloned in plasmids in Escherichia coli despite strong expression repression. Finally, site-directed insertion of smrA and satAB in the amy locus of the Bacillus subtilis chromosome using ligated PCR amplicons allowed for the functional expression and study of both pumps. Results showed that SatAB is a narrow-spectrum fluoroquinolone exporter (norfloxacin and ciprofloxacin), susceptible to reserpine, whereas SmrA was not involved in fluoroquinolone resistance. Chromosomal integration in Bacillus is a novel method for studying efflux pumps from Gram-positive bacteria, which enabled us to demonstrate the possible role of SatAB, and not SmrA, in fluoroquinolone efflux in S. suis.
PB American Society for Microbiology
SN 1098-6596
YR 2011
FD 2011-12
LK https://hdl.handle.net/20.500.14352/45177
UL https://hdl.handle.net/20.500.14352/45177
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Comunidad de Madrid
DS Docta Complutense
RD 6 abr 2025