RT Journal Article
T1 A comprehensive map of microbial biomarkers along the gastrointestinal tract for celiac disease patients
A1 Arcila-Galvis, Juliana Estefanía
A1 Loria Kohen, Viviana Constanza
A1 Ramírez de Molina, Ana
A1 Carrillo de Santa Pau, Enrique
A1 Marcos-Zambrano, Laura Judith
AB Dysbiosis of the microbiome has been related to Celiac disease (CeD) progress, an autoimmune disease characterized by gluten intolerance developed in genetically susceptible individuals under certain environmental factors. The microbiome contributes to CeD pathophysiology, modulating the immune response by the action of short-chain fatty acids (SCFA), affecting gut barrier integrity allowing the entrance of gluten-derived proteins, and degrading immunogenic peptides of gluten through endoprolyl peptidase enzymes. Despite the evidence suggesting the implication of gut microbiome over CeD pathogenesis, there is no consensus about the specific microbial changes observed in this pathology. Here, we compiled the largest dataset of 16S prokaryotic ribosomal RNA gene high-throughput sequencing for consensus profiling. We present for the first time an integrative analysis of metataxonomic data from patients with CeD, including samples from different body sites (saliva, pharynx, duodenum, and stool). We found the presence of coordinated changes through the gastrointestinal tract (GIT) characterized by an increase in <jats:italic>Actinobacteria</jats:italic> species in the upper GIT (pharynx and duodenum) and an increase in <jats:italic>Proteobacteria</jats:italic> in the lower GIT (duodenum and stool), as well as site-specific changes evidencing a dysbiosis in patients with CeD’ microbiota. Moreover, we described the effect of adherence to a gluten-free diet (GFD) evidenced by an increase in beneficial bacteria and a decrease in some <jats:italic>Betaproteobacteriales</jats:italic> but not fully restoring CeD-related dysbiosis. Finally, we built a Random Forest model to classify patients based on the lower GIT composition achieving good performance.
PB Frontiers
SN 1664-302X
YR 2022
FD 2022-09-13
LK https://hdl.handle.net/20.500.14352/104160
UL https://hdl.handle.net/20.500.14352/104160
LA eng
NO Arcila-Galvis JE, Loria-Kohen V, Ramírez de Molina A, Carrillo de Santa Pau E, Marcos-Zambrano LJ. A comprehensive map of microbial biomarkers along the gastrointestinal tract for celiac disease patients. Front Microbiol. 2022 Sep 13;13:956119. doi: 10.3389/fmicb.2022.956119.
NO European Society of Clinical Microbiology and Infectious Diseases (ESCMID)
NO Convocatoria FEDER INNTERCONECTA 2015
NO Juan de la Cierva
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 7 abr 2025