RT Journal Article
T1 Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines
A1 Arévalo Pérez, Beatriz
A1 Blázquez García, Marina
A1 Valverde De La Fuente, Alejandro
A1 Serafín González-Carrato, Verónica
A1 Montero Calle, Ana
A1 Solís Fernández, Guillermo
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarron, José M.
AB A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specifc antibodies (cAbs) and a strategy for signal amplifcation based on labelling the detection antibodies (dAbs) with binary MoS2/ MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL−1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
PB Springer
SN 0026-3672
YR 2022
FD 2022-03-14
LK https://hdl.handle.net/20.500.14352/71579
UL https://hdl.handle.net/20.500.14352/71579
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto de Salud Carlos III (ISCIII)
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (MICINN)
NO Flanders Research Foundation (FWO)
DS Docta Complutense
RD 15 abr 2025