RT Conference Proceedings
T1 Persistence of Legionella pneumophila serogroup 1 into water system installations
A1 García Esteban, María Teresa
A1 Baladrón, B.
A1 Pelaz, C.
AB Legionella pneumophila serogroup 1 is the principal agent of Legionnaires' disease (LD) and the more frequently isolated from the enviroment. Persistence of the bacteria into water system installations has been demostrated in LD hospital associated which has been disinfected several times. Objectives. To compare the molecular pattern of different epidemiological markers and the susceptibility to disinfectants of L. pneumophila serogroup 1 Pontiac strains, recovered along several years of hot water system installations of 3 LD building associated. Methods. We compare the genetic patterns of several L. pneumophila serogroup 1 environmental isolates recovered from LD building associated (a hotel, a hospital and a ship), which were hiperclhorinated several times. We use AFLP, PFGE and AP-PCR as the molecular methods. Susceptibility of these strains against five disinfectants (chlorine and 4 products registered in thePlaguicides Register of Spanish Health Ministery) by determination of MIC and MBC, was also compared. Results. Identical AFLP, PFGE and AP-PCR pattern and similar MIC and MBC values, were detected for all strains recovered from the same building along the years. For each disinfectant MIC50 and MIC0 values were similar: 2-4 ppm for Neoseptal KK, 8-16 ppm for Spectrus NX 1102, 256 ppm for Sanosil Super 25, 128-512 for Bioguimon and 1.024 ppm forchlorine. The CMB values increased no more than 4 twofold dilutions with respect to CMI values. Conclusions. A clon of L. pneumophila serogroup 1 Pontiac persisted along the years in the water system installation of 3 different LD building associated, in spite of successive hiperchlorinations. Susceptibility of each clon to disinfectants did not change, in spite of these treatments. All disinfectants showed bactericidal and inhibitory activity. Chlorine was the lest effective.
YR 2003
FD 2003
LK https://hdl.handle.net/20.500.14352/130343
UL https://hdl.handle.net/20.500.14352/130343
LA eng
DS Docta Complutense
RD 17 ene 2026