%0 Journal Article
%A Valverde De La Fuente, Alejandro
%A Montero Calle, Ana
%A Arévalo Pérez, Beatriz
%A San Segundo Acosta, Pablo
%A Serafín González-Carrato, Verónica
%A Alonso Navarro, Miren
%A Solís Fernández, Guillermo
%A Pingarrón Carrazón, José Manuel
%A Campuzano Ruiz, Susana
%A Barderas Manchado, Rodrigo
%T Phage-Derived and Aberrant HaloTag PeptidesImmobilized on Magnetic Microbeads for AmperometricBiosensing of Serum Autoantibodies and Alzheimer'sDisease Diagnosis
%D 2021
%@ 2629-2742
%U https://hdl.handle.net/20.500.14352/4685
%X An electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100% obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.
%~