RT Journal Article
T1 Phage-Derived and Aberrant HaloTag PeptidesImmobilized on Magnetic Microbeads for AmperometricBiosensing of Serum Autoantibodies and Alzheimer'sDisease Diagnosis
A1 Valverde De La Fuente, Alejandro
A1 Montero Calle, Ana
A1 Arévalo Pérez, Beatriz
A1 San Segundo Acosta, Pablo
A1 Serafín González-Carrato, Verónica
A1 Alonso Navarro, Miren
A1 Solís Fernández, Guillermo
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
A1 Barderas Manchado, Rodrigo
AB An electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100% obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.
PB Wiley-VCH
SN 2629-2742
YR 2021
FD 2021-07-27
LK https://hdl.handle.net/20.500.14352/4685
UL https://hdl.handle.net/20.500.14352/4685
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2021)
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto de Salud Carlos III (ISCIII)
NO Comunidad de Madrid
DS Docta Complutense
RD 7 abr 2025