RT Journal Article
T1 The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana
A1 Feng, Chao
A1 Lorenz, Jana
A1 Dreissig, Steven
A1 Schubert, Veit
A1 Wang, Baicui
A1 Hartmann, Franziska
A1 Cuacos, Maria
A1 Fernández Jiménez, Nadia
A1 Zhao, Ziliang
A1 Eggeling, Christian
A1 Câmara, Amanda Souza
A1 Himmelbach, Axel
A1 Heckmann, Stefan
AB The synaptonemal complex (SC) forms between homologous chromosomes during meiosis. In Arabidopsis thaliana, its central region (CR) is composed of the transverse filament protein ZYP1 and the central element proteins SCEP1 and SCEP2. Here we identify SCEP3 as a CR protein that is evolutionarily conserved across plant species. SCEP3 spatiotemporally overlaps with other CR proteins and localizes to the SC CR. The loss of SCEP3 prevents SC assembly, abolishes crossover (CO) assurance and interference, and eliminates sex-specific differences in CO rates (heterochiasmy) through increased CO in females. SCEP3 is required for a subset of COs in SC-deficient mutants, such as zyp1. Although SCEP3 physically interacts with ZYP1, it loads independently of other CR proteins. We propose that SCEP3 may associate with certain recombination intermediates, stabilizing them and/or recruiting additional factors, such as ZYP1, to a subset of these intermediates, thereby promoting and interlinking SC assembly and CO formation.
PB Springer
SN 2055-0278
YR 2025
FD 2025-06
LK https://hdl.handle.net/20.500.14352/123155
UL https://hdl.handle.net/20.500.14352/123155
LA eng
NO Feng, C., Lorenz, J., Dreissig, S. et al. The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana. Nat. Plants 11, 1353–1366 (2025). https://doi.org/10.1038/s41477-025-02030-9
NO Open access funding provided by Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK).
NO Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
DS Docta Complutense
RD 10 ago 2025