RT Journal Article
T1 Phylogeography of organophosphate resistant ace alleles in spanish olive fruit fly populations: a mediterranean perspective in the global change context
A1 Lantero Bringas, Esther
A1 Matallanas Peñas, Beatriz
A1 Pascual, Susana
A1 Ochando González, María Dolores
A1 Callejas Hervás, Carmen
AB The olive fruit fly (olf) Bactrocera oleae is the most damaging olive pest. The intensive use of organophosphates (OPs) to control it, led to an increase in resistance in field populations. This study assesses the presence and distribution of three mutations at the ace gene related to target site insensitivity to OPs in Spain. Samples from other Mediterranean countries were included as external references. Resistance-conferring alleles (from exons IV and VII of the ace gene) reached almost an 80% frequency in olf Spanish populations. In total, 62% of them were homozygous (RR/RR), this being more common in eastern mainland Spain. High frequencies of RR/RR individuals were also found in North Mediterranean samples. Conversely, in Tunisia, only sensitive alleles were detected. Finally, the exon X mutation ∆Q3 had an extremely low frequency in all samples. The high frequency of genotype RR/RR in Spain indicates high fitness in an agroecosystem treated with pesticides, in contrast to ∆Q3. At exon IV all flies carried the same haplotype for the allele conferring resistance. The sequence analysis at this exon suggests a unique origin and fast expansion of the resistant allele. These results provide evidence that OPs appropriate use is needed and prompt the search for alternative methods for olf pest control.
PB MDPI
SN ESSN 2075-4450
YR 2020
FD 2020-06-26
LK https://hdl.handle.net/20.500.14352/6615
UL https://hdl.handle.net/20.500.14352/6615
LA eng
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 12 abr 2025