RT Journal Article
T1 Electrochemical magnetic beads-based immunosensing platform for the determination of α-lactalbumin in milk
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Campuzano Ruiz, Susana
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Reviejo García, Ángel Julio
A1 Pingarrón Carrazón, José Manuel
AB Alpha-lactalbumin (α-LA) is one of the whey proteins in cows’ milk that has been identified as allergenic. In this work, we present, for the first time, a very sensitive magnetic beads (MBs)-based immunosensor for the determination of α-LA. A sandwich configuration involving selective capture and horseradish peroxidase-labeled detector antibodies was implemented on carboxylic acid-modified magnetic beads, captured magnetically under the surface of a disposable screen-printed carbon electrode for amperometric detection using the hydroquinone (HQ)/hydrogen peroxide system. The α-LA immunosensor exhibited a wide linear range (37.0–5000 pg/ml), a low limit of detection (LOD, 11.0 pg/ml) and noteworthy selectivity against other non-target proteins. The MBs-based immunosensing platform was applied successfully for the determination of α-LA in several varieties of milk (raw and UHT cows’ milk as well as human milk) and infant formulations. The results were corroborated with those obtained using a commercial ELISA method, thereby substantiating the analytical merits of this unique method.
PB Elsevier BV
SN 0308-8146
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/95452
UL https://hdl.handle.net/20.500.14352/95452
LA eng
NO Ruiz-Valdepeñas Montiel V, Campuzano S, Torrente-Rodríguez RM, Reviejo AJ, Pingarrón JM. Electrochemical magnetic beads-based immunosensing platform for the determination of α-lactalbumin in milk. Food Chem. 2016 Dec 15;213:595-601. doi: 10.1016/j.foodchem.2016.07.004. Epub 2016 Jul 5. PMID: 27451223.
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 7 jul 2025