RT Journal Article
T1 Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction
A1 Prat Vidal, Cristina
A1 Crisóstomo, Verónica
A1 Moscoso, Isabel
A1 Báez Díaz, Claudia
A1 Blanco Blázquez, Virginia
A1 Gómez Mauricio, Guadalupe
A1 Albericio, Guillermo
A1 Aguilar, Susana
A1 Fernández Santos, María Eugenia
A1 Fernández Avilés, Francisco
A1 Sánchez Margallo, Francisco M.
A1 Bayes Genis, Antoni
A1 Bernad, Antonio
AB Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFPpos and HGF-mCherrypos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 106/animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function.
PB MPDI
SN 2073-4409
YR 2021
FD 2021-12-28
LK https://hdl.handle.net/20.500.14352/71433
UL https://hdl.handle.net/20.500.14352/71433
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Ministerio de Economía y Competitividad (MINECO)
NO Instituto de Salud Carlos III (ISCIII)
DS Docta Complutense
RD 5 abr 2025