RT Journal Article
T1 Unravelling the Role of Candida albicans Prn1 in the Oxidative Stress Response through a Proteomics Approach
A1 Arribas Antón, Víctor
A1 Monteoliva Díaz, Lucía
A1 Hernáez Sánchez, María Luisa
A1 Gil, Concha
A1 Molero Martín-Portugués, María Gloria
AB Abstract: Candida albicans Prn1 is a protein with an unknown function similar to mammalian Pirin. It also has orthologues in other pathogenic fungi, but not in Saccharomyces cerevisiae. Prn1 highly increases its abundance in response to H2O2 treatment; thus, to study its involvement in the oxidative stress response, a C. albicans prn1D mutant and the corresponding wild-type strain SN250 have been studied. Under H2O2 treatment, Prn1 absence led to a higher level of reactive oxygen species (ROS) and a lower survival rate, with a higher percentage of death by apoptosis, confirming its relevant role in oxidative detoxication. The quantitative differential proteomics studies of both strains in the presence and absence of H2O2 indicated a lower increase in proteins with oxidoreductase activity after the treatment in the prn1D strain, as well as an increase in proteasome-activating proteins, corroborated by in vivo measurements of proteasome activity, with respect to the wild type. In addition, remarkable differences in the abundance of some transcription factors were observed between mutant and wild-type strains, e.g., Mnl1 or Nrg1, an Mnl1 antagonist. orf19.4850, a protein orthologue to S. cerevisiae Cub1, has shown its involvement in the response to H2O2 and in proteasome function when Prn1 is highly expressed in the wild type.
PB MDPI
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/126230
UL https://hdl.handle.net/20.500.14352/126230
LA eng
NO Arribas, V.; Monteoliva, L.; Hernáez, M.L.; Gil, C.; Molero, G. Unravelling the Role of Candida albicans Prn1 in the Oxidative Stress Response through a Proteomics Approach. Antioxidants 2024, 13, 527. https://doi.org/10.3390/ antiox13050527
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 31 dic 2025