RT Journal Article
T1 Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production
T2 Ingeniería metabólica de Rhodococcus ruber Chol-4: Una factoría celular para la producción de testosterona
A1 Guevara Acosta, Flor Govinda
A1 Olortegui Flores, Yamileth
A1 Fernández de las Heras, Laura
A1 Perera González, Julián
A1 Navarro Llorens, Juana María
AB Rhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of the steroid catabolism inside the strain. A R. ruber quadruple mutant was constructed in order to avoid the breakage of the steroid nucleus. At the same time, an inducible expression vector for this strain was developed. The 17-ketoreductase gene from the fungus Cochliobolus lunatus was cloned and overexpressed in this vector. The engineered strain was able to produce testosterone from 4-androstene-3,17-dione using glucose for cofactor regeneration with a molar conversion of 61%. It is important to note that 91% of the testosterone was secreted outside the cell after 3 days of cell biotransformation. The results support the idea that Rhodococcus ruber Chol-4 can be metabolically engineered and can be used for the production of steroid intermediates.
PB Public Library of Science
SN 1932-6203
YR 2019
FD 2019-07-26
LK https://hdl.handle.net/20.500.14352/13790
UL https://hdl.handle.net/20.500.14352/13790
LA eng
NO Guevara G, Olortegui Flores Y, Fernández de Las Heras L, Perera J, Navarro Llorens JM. Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production. PLoS One. 2019 Jul 26;14(7):e0220492. doi: 10.1371/journal.pone.0220492. PMID: 31348804; PMCID: PMC6660089.
NO Ministerio de Economía, Comercio y Empresa (España)
DS Docta Complutense
RD 14 ago 2024