RT Journal Article
T1 Detection of Androgen Receptors in Spermatozoa of Small Ruminants: A Putative Modulation Pathway for Cryoresistance Through AQP3
A1 Alba, Esther
A1 Castaño, Cristina
A1 Toledano Díaz, Adolfo
A1 Velázquez, Rosario
A1 Martínez Madrid, Carmen Belén
A1 Gómez Crespo, Alberto
A1 Álvarez Rodríguez, Manuel
A1 Rodriguez Martinez, Heriberto
A1 Santiago Moreno, Julián
AB This work was aimed to identify androgen receptors (AR) in the spermatozoa of wild and domestic ruminants and to assess the effect of testosterone on sperm localization of aquaporin-3 (AQP3) and cryopreservation process. Sperm samples from wild species were incubated with testosterone (T group), 1,3-propanediol (PDO group), phloretin (PHL group), PDO+T group, PHL+T group. Western blot identified the presence of AR as a single band of about 48 KDa. Immunolabelling of AR was located in the equatorial segment of the sperm head. In mouflons, the cryoresistance ratio for acrosome integrity was lower (p < 0.05) in the PHL+T than in Control and T groups. In ibexes, the cryoresistance ratio for acrosome integrity was lower (p < 0.05) in the PHL+T, PHL, and T group than in the Control group; the cryoresistance ratios for sperm kinematic variables were lower (p < 0.05) in PDO+T than in Control. No changes were found among treatments in the proportion of spermatozoa showing AQP3 in the different membrane domains after incubation and thawing in both mouflon and ibex. In conclusion, testosterone negatively affected sperm cryoresistance expressed as acrosome integrity, enhancing the effects of the AQP blocker PHL. Our findings provide a sound knowledge of the molecular mechanisms that explain the seasonal variation in sperm freezability from ruminants.
PB MDPI
SN 16616596
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/112351
UL https://hdl.handle.net/20.500.14352/112351
LA eng
NO Alba, E., Castaño, C., Toledano-Díaz, A., Velázquez, R., Martínez-Madrid, B., Gómez-Crespo, A., Álvarez-Rodríguez, M., Rodriguez-Martinez, H., & Santiago-Moreno, J. (2024). Detection of Androgen Receptors in Spermatozoa of Small Ruminants: A Putative Modulation Pathway for Cryoresistance Through AQP3. International journal of molecular sciences, 25(22), 11972. https://doi.org/10.3390/ijms252211972
NO Author Contributions: Conceptualization, J.S.-M.; methodology, E.A., C.C., A.T.-D., R.V. and A.G.-C.;formal analysis, E.A., C.C., A.T.-D., R.V., A.G.-C. and J.S.-M.; investigation M.Á.-R. and H.R.-M.;resources, J.S.-M.; writing—original draft preparation, E.A.; writing—J.S.-M., B.M.-M., M.Á.-R. andH.R.-M.; review and editing, E.A., C.C., A.T.-D., R.V., B.M.-M., M.Á.-R. and H.R.-M.; supervision,J.S.-M.; project administration, J.S.-M.; funding acquisition, J.S.-M. All authors have read and agreedto the published version of the manuscript
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Consejo Superior de Investigaciones Científicas (España)
NO Instituto de Agrobiotecnología ( España)
DS Docta Complutense
RD 17 abr 2025