TY - JOUR AU - Chinchilla Rodríguez, Blanca AU - Encinas, Paloma AU - Estepa, Amparo AU - Coll, Julio AU - Gómez Casado, Eduardo PY - 2013 DO - 10.1016/j.jviromet.2013.07.049 SN - 0166-0934 UR - https://hdl.handle.net/20.500.14352/97352 T2 - Journal of Virological Methods AB - A new high throughput centrifugation-free method to estimate viral neutralizing antibody levels in low volumes and large numbers of plasma blood samples is described. Cell monolayers were, (i) plated on poly-D-Lys coated 96-wells, (ii) infected with... LA - eng M2 - 627 PB - Elsevier KW - Fixed-permeabilized monolayers KW - VHSV KW - Zebrafish KW - Highthroughput KW - Neutralizing antibodies KW - Assay TI - Optimization of fixed-permeabilized cell monolayers for high throughput micro-neutralizing antibody assays: application to the zebrafish/viral hemorrhagic septicemia virus (vhsv) model TY - journal article VL - 193 ER -