RT Journal Article
T1 Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds
A1 Gómez Buendía, Alberto
A1 Álvarez Sánchez, Julio
A1 Bezos Garrido, Javier
A1 Mourelo, Jorge
A1 Amado, Javier
A1 Saez, José Luis
A1 Juan Ferré, Lucía De
A1 Romero Martínez, Beatriz
AB Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collection of 373 isolates obtained from skin test positive cows from 359 officially tuberculosis-free (OTF) herds, culled in the framework of the bTB eradication campaign in Spain, were identified at the species level through PCR and Sanger sequencing of the 16S rDNA, hsp65 and rpoB genes.. Of the 308 isolates for which a reliable identification was achieved, 32 different mycobacterial species were identified, with certain species being most represented: among M. avium complex members (n = 142, 46.1%), M. avium subsp. hominissuis (98; 69.0%) was the most abundant followed by M. avium subsp. avium (33, 23.2%), and M. intracellulare(7, 4.9%). Among non-MAC members (n = 166, 53.9%), M. nonchromogenicum (85; 27.6%) and M. bourgelatii (11; 5.6%) were the predominant species. In addition, mixed results were obtained in 53 isolates presenting up to 30 different genotypes, which could be indicative of new mycobacterial species. Our results represent a first step toward characterizing the diversity of NTM species that could interfere with official diagnostic tests for bTB eradication in Spain.
PB Frontiers
SN 2297-1769
YR 2024
FD 2024-01-31
LK https://hdl.handle.net/20.500.14352/102515
UL https://hdl.handle.net/20.500.14352/102515
LA eng
NO Gómez-Buendía A, Alvarez J, Bezos J, Mourelo J, Amado J, Saez-Llorente JL, de Juan L and Romero B*. Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds. Frontiers in veterinary science, 11:1361788. 2024. (A). ISSN: 2297-1769. Impact factor 2022: 3.200. Category: Veterinary Sciences, Quartile: 1, Position: 12 of 143. DOI: 10.3389/fvets.2024.1361788
NO Author contributions: AG-B: Data curation, Formal analysis, Writing—original draft, Writing—review & editing. JAl: Data curation, Funding acquisition, Resources, Supervision, Writing—review & editing. JB: Funding acquisition, Writing—review & editing. JM: Conceptualization, Supervision, Writing—review & editing. JAm: Data curation, Resources, Writing—review & editing. JS: Investigation, Resources, Writing—review & editing. LdJ: Data curation, Funding acquisition, Writing—review & editing. BR: Data curation, Methodology, Supervision, Writing—review & editing.
NO European Union Reference Laboratory for Bovine Tuberculosis
NO Ministerio de Agricultura, Pesca y Alimentación
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 8 abr 2025