RT Journal Article
T1 CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
A1 Fernández Prieto, Marta
A1 Núñez, Concepción
A1 Fernández Aceñero, María Jesús
A1 López Palacios, Natalia
A1 Bodas Pinedo, Andrés
A1 Farrais, Sergio
A1 Cuevas, David
A1 Pascual, Virginia
A1 Cerón Nieto, María Ángeles
A1 Horta Herrera, Saúl
A1 Espino Paisán, Laura
A1 Salazar Mendoza, Isabel
AB Background: The CX3CL1–CX3CR1 axis has been related to numerous diseases. The aim of our study was to investigate its involvement in coeliac disease (CD) pathogenesis, particularly in the early phase of the disease. Methods: We collected peripheral blood from CD patients and controls, enrolled in a 3-day gluten challenge, to study soluble CX3CL1, I-TAC and MIG by Luminex, CX3CL1 and CX3CR1 gene expression by qPCR, and CX3CR1 protein expression in monocytes and CD8+, CD4+ and γδ+ T cells, by flow cytometry. We also analysed the expression of the CX3CL1 and CX3CR1 mRNA and protein in the duodenal biopsies of CD patients with active and treated disease, and in non-CD control individuals, by qPCR and immunohistochemistry. Results: After the gluten challenge, increased levels of CX3CL1, I-TAC and MIG proteins were observed in the peripheral blood of CD patients, with no changes in CX3CL1 mRNA, or CX3CR1 mRNA and protein. Regarding duodenal tissue, CX3CL1 was absent or barely present in the superficial and basal epithelium of CD patients, contrasting with the moderate to high levels present in controls. Conclusions: CX3CL1 seems to be involved in the appearance and progression of CD, and it appears to be a potential diagnostic biomarker. Its use as an alternative therapeutic target in CD deserves further research.
PB MDPI
SN 2072-6643
YR 2019
FD 2019-10-23
LK https://hdl.handle.net/20.500.14352/12534
UL https://hdl.handle.net/20.500.14352/12534
LA eng
NO Fernández Prieto, M., Fernández Aceñero, M. J., López Palacios, N. et al. «CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis». Nutrients, vol. 11, n.o 11, octubre de 2019, p. 2551. DOI.org (Crossref), https://doi.org/10.3390/nu11112551.
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
DS Docta Complutense
RD 7 abr 2025