RT Journal Article
T1 Developing an efficient strategy for immobilizationof PHB depolymerase on magnetite-based nanoparticles for degrading polyhydroxybutyrate in acidic conditions
A1 García Hidalgo, Javier
A1 Acebal Sarabia, Carmen
A1 De La Mata Riesco, Mª Isabel
A1 Arroyo Sánchez, Miguel
AB Several approaches towards the efficient immobilization of poly-3-hydroxybutyrate (PHB) depolymerase from Streptomyces ascomycinicus (PhaZSa) on magnetic-silanized nanoparticles have been explored in order to accomplish the preparation of an active immobilized biocatalyst in PHB hydrolysis in acid conditions. A simple non-covalent adsorption of PhaZSa to silanized magnetic particles at acidic pH during a short incubation time allowed the preparation of immobilized biocatalysts with increased specific activity. In contrast, covalent binding of PhaZSa to silanized magnetite by different methods led to immobilized biocatalysts whose retained activity were low or void.
PB Taylor & Francis
SN 1024-2422, ESSN: 1029-2446
YR 2017
FD 2017-05
LK https://hdl.handle.net/20.500.14352/18037
UL https://hdl.handle.net/20.500.14352/18037
LA eng
NO Unión Europea. FP6
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 10 abr 2025