RT Journal Article
T1 Determination of ibuprofen enantiomers in breast milk using vortex-assisted matrix solid-phase dispersion and direct chiral liquid chromatography
A1 León González, María Eugenia
A1 Rosales Conrado, Noelia
AB A mixture of B-cyclodextrin (B-CD) and primary and secondary amine (PSA) sorbents was employed for the extraction and quantification of ibuprofen enantiomers from human breast milk, combining a vortex-assisted matrix solid-phase dispersion method (MSPD) and direct chiral liquid chromatography (CLC) with ultraviolet detection (UV). The MSPD sample preparation procedure was optimized focusing on both the type and amount of dispersion/sorption sorbents and the nature of the elution solvent, in order to obtain acceptable recoveries and avoiding enantiomer conversion. These MSPD parameters were optimized with the aid of an experimental design approach. Hence, a factorial design was used for identification of the main variables affecting the extraction process of ibuprofen enantiomers. Under optimum selected conditions, MSPD combined with direct CLC-UV was successfully applied for ibuprofen enantiomeric determination in breast milk at enantiomer levels between 0.15 and 6.0 µg∙g-1. The proposed analytical method also provided good repeatability, with relative standard deviations of 6.4 % and 8.3 % for the intra-day and inter-day precision, respectively.
PB Elsevier
SN 0021-9673, 1873-3778
YR 2017
FD 2017-09
LK https://hdl.handle.net/20.500.14352/18323
UL https://hdl.handle.net/20.500.14352/18323
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid/FEDER
DS Docta Complutense
RD 29 abr 2024