RT Journal Article
T1 A Novel Lipase from Streptomyces exfoliatus DSMZ 41693 for Biotechnological Applications
A1 Rodríguez-Alonso, Guillermo
A1 Toledo-Marcos, Juan
A1 Serrano-Aguirre, Lara
A1 Rumayor, Carlos
A1 Pasero, Beatriz
A1 Flores Aguilar-Amat, Aída
A1 Saborido Modia, Ana Isolina
A1 Hoyos Vidal, María Pilar
A1 Hernáiz Gómez-Degano, María Josefa
A1 De La Mata Riesco, Mª Isabel
A1 Arroyo Sánchez, Miguel
AB Genome mining of Streptomyces exfoliatus DSMZ 41693 has allowed us to identify four different lipase-encoding sequences, and one of them (SeLipC) has been successfully cloned and extracellularly expressed using Rhodococcus sp. T104 as a host. SeLipC was purified by one-step hydrophobic interaction chromatography. The enzyme is a monomeric protein of 27.6 kDa, which belongs to subfamily I.7 of lipolytic enzymes according to its phylogenetic analysis and biochemical characterization. The purified enzyme shows the highest activity at 60 °C and an optimum pH of 8.5, whereas thermal stability is significantly improved when protein concentration is increased, as confirmed by thermal deactivation kinetics, circular dichroism, and differential scanning calorimetry. Enzyme hydrolytic activity using p-nitrophenyl palmitate (pNPP) as substrate can be modulated by different water-miscible organic cosolvents, detergents, and metal ions. Likewise, kinetic parameters for pNPP are: KM = 49.6 µM, kcat = 57 s−1, and kcat/KM = 1.15 × 106 s−1·M−1. SeLipC is also able to hydrolyze olive oil and degrade several polyester-type polymers such as poly(butylene succinate) (PBS), poly(butylene succinate)-co-(butylene adipate) (PBSA), and poly(ε-caprolactone) (PCL). Moreover, SeLipC can catalyze the synthesis of different sugar fatty acid esters by transesterification using vinyl laurate as an acyl donor, demonstrating its interest in different biotechnological applications.
PB MDPI
SN 1422-0067
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/91305
UL https://hdl.handle.net/20.500.14352/91305
LA eng
NO Rodríguez-Alonso, G.; Toledo-Marcos, J.; Serrano-Aguirre, L.; Rumayor, C.; Pasero, B.; Flores, A.; Saborido, A.; Hoyos, P.; Hernáiz, M.J.; de la Mata, I.; et Arroyo, M (2023) "A Novel Lipase from Streptomyces exfoliatus DSMZ 41693 for Biotechnological Applications" Int. J. Mol. Sci. 24, no. 23: 17071. https://doi.org/10.3390/ijms242317071
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO European Commission
DS Docta Complutense
RD 6 abr 2025