RT Journal Article
T1 Emerging, Non-PCV13 Serotypes 11A and 35B of Streptococcus pneumoniae Show High Potential for Biofilm Formation In Vitro
A1 Domenech Lucas, Miriam
A1 Damián, Diana
A1 Ardanuy, Carmen
A1 Liñares, Josefina
A1 Fenoll, Asunción
A1 García, Ernesto
AB Since the use of pneumococcal conjugate vaccines PCV7 and PCV13 in children became widespread, invasive pneumococcal disease (IPD) has dramatically decreased. Nevertheless, there has been a rise in incidence of Streptococcus pneumoniae non-vaccine serotypes (NVT) colonising the human nasopharynx. Nasopharyngeal colonisation, an essential step in the development of S. pneumoniae-induced IPD, is associated with biofilm formation. Although the capsule is the main pneumococcal virulence factor, the formation of pneumococcal biofilms might, in fact, be limited by the presence of capsular polysaccharide (CPS).Methodology/Principal FindingsWe used clinical isolates of 16 emerging, non-PCV13 serotypes as well as isogenic transformants of the same serotypes. The biofilm formation capacity of isogenic transformants expressing CPSs from NVT was evaluated in vitro to ascertain whether this trait can be used to predict the emergence of NVT. Fourteen out of 16 NVT analysed were not good biofilm formers, presumably because of the presence of CPS. In contrast, serotypes 11A and 35B formed ≥45% of the biofilm produced by the non-encapsulated M11 strain.Conclusions/SignificanceThis study suggest that emerging, NVT serotypes 11A and 35B deserve a close surveillance. Since the use of pneumococcal conjugate vaccines PCV7 and PCV13 in children became widespread, invasive pneumococcal disease (IPD) has dramatically decreased. Nevertheless, there has been a rise in incidence of Streptococcus pneumoniae non-vaccine serotypes (NVT) colonising the human nasopharynx. Nasopharyngeal colonisation, an essential step in the development of S. pneumoniae-induced IPD, is associated with biofilm formation. Although the capsule is the main pneumococcal virulence factor, the formation of pneumococcal biofilms might, in fact, be limited by the presence of capsular polysaccharide (CPS).Methodology/Principal FindingsWe used clinical isolates of 16 emerging, non-PCV13 serotypes as well as isogenic transformants of the same serotypes. The biofilm formation capacity of isogenic transformants expressing CPSs from NVT was evaluated in vitro to ascertain whether this trait can be used to predict the emergence of NVT. Fourteen out of 16 NVT analysed were not good biofilm formers, presumably because of the presence of CPS. In contrast, serotypes 11A and 35B formed ≥45% of the biofilm produced by the non-encapsulated M11 strain.Conclusions/SignificanceThis study suggest that emerging, NVT serotypes 11A and 35B deserve a close surveillance.
PB Public Library of Science
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/97608
UL https://hdl.handle.net/20.500.14352/97608
LA eng
NO Domenech M, Damián D, Ardanuy C, Liñares J, Fenoll A, García E (2015) Emerging, Non-PCV13 Serotypes 11A and 35B of Streptococcus pneumoniae Show High Potential for Biofilm Formation In Vitro. PLoS ONE 10(4): e0125636. https://doi.org/10.1371/journal.pone.0125636
NO Ministerio de Economía y Competitividad (España)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 2 oct 2024